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Image Search Results
Journal: Frontiers in Neuroscience
Article Title: Dystrophin Is Required for the Proper Timing in Retinal Histogenesis: A Thorough Investigation on the mdx Mouse Model of Duchenne Muscular Dystrophy
doi: 10.3389/fnins.2020.00760
Figure Lengend Snippet: Primary antibodies used for immunofluorescence.
Article Snippet:
Techniques: Immunofluorescence
Journal: eNeuro
Article Title: Demyelination and Na + Channel Redistribution Underlie Auditory and Vestibular Dysfunction in PMP22-Null Mice
doi: 10.1523/ENEURO.0462-23.2023
Figure Lengend Snippet: Afferent type I SGN subtype cell bodies are intact in 3-week-old PMP22 −/− mice. A , B , Photomicrographs of a section through PMP22 +/+ and PMP22 −/− inner ear show calbindin (Calb1)-positive neurons (red) in the spiral and vestibular ganglion (aka, Scarpa ganglion). Neurons were stained with Tuj1 (green). Scale bar, 10 µm. C – E , Summary data of “blind” neuronal counts by three individuals. Similar analyses were performed for calretinin (Calb2) and Pou4f1-positive neurons. F – H , Evaluation of Calb1-, Calb2-, and Pou4f1-positive VNs in the Scarpa ganglion. The two genotypes had no statistical differences in the indicated neuronal counts ( n = 9; mean samples from 25 sections from 4 mice).
Article Snippet: Microdissected pieces were immunostained with antibodies to the following: (1) mouse anti-C-terminal binding protein 2 (presynaptic marker, BD Biosciences, 1:200); (2) rabbit anti-myosin VIIa (HC marker, Proteus Biosciences, 1:600); (3) rabbit anti-Homer 1 (postsynaptic marker, Synaptic Systems, 1:250); (4) rabbit anti-Tuj1 (BioLegend, 1:500), chicken anti-Tuj1 (Abcam,1:500), and mouse anti-Tuj1 (Abcam, 1:500); (5) rabbit anti-PMP22 (Abcam, 1:500); (6) chicken anti-MBP (EnCor Biotechnology, 1:500), rabbit anti-MBP (Abcam, 1:500), mouse IgG2b anti-MBP (Abcam, 1:500); (7) chicken anti-MPZ (Abcam, 1:500); (8)
Techniques: Staining